Probing Protein-Lipid Interactions at the Single Molecule Level

Probing transient copper chaperone-Wilson disease protein interactions at the single-molecule level with nanovesicle trapping.

the Single-Molecule Level with Nanovesicle Trapping Jaime J. Benı́tez,† Aaron M. Keller,† Patrick Ochieng,‡ Liliya A. Yatsunyk,§ David L. Huffman,*,‡ Amy C. Rosenzweig,§ and Peng Chen*,† Department of Chemistry and Chemical Biology, Cornell UniVersity, Ithaca, New York 14853, Department of Chemistry, Western Michigan UniVersity, Kalamazoo, Michigan 49008, and Departments of Biochemistry, Molecul...

Visualizing protein-DNA interactions at the single-molecule level.

Recent advancements in single-molecule methods have allowed researchers to directly observe proteins acting on their DNA targets in real-time. Single-molecule imaging of protein-DNA interactions permits detection of the dynamic behavior of individual complexes that otherwise would be obscured in ensemble experiments. The kinetics of these processes can be monitored directly, permitting identifi...

Probing Intranuclear Environments at the Single-Molecule Level

Genome activity and nuclear metabolism clearly depend on accessibility, but it is not known whether and to what extent nuclear structures limit the mobility and access of individual molecules. We used fluorescently labeled streptavidin with a nuclear localization signal as an average-sized, inert protein to probe the nuclear environment. The protein was injected into the cytoplasm of mouse cell...

Probing DNA-peptide interaction forces at the single-molecule level.

The versatility of chemical peptide synthesis combined with the high sensitivity of AFM single-molecule force spectroscopy allows us to investigate, quantify, and control molecular recognition processes (molecular nanotechnology), offering a tremendous potential in chemical biology.Single-molecule force spectroscopy experiments are able to detect fast intermediate transition states, details of ...

Probing protein-chaperone interactions with single-molecule fluorescence spectroscopy.